FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12069137
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC282140.23376982132193874No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA279770.23180613493740274No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG273180.22634592680487428No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT225960.18722133985221975No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG215930.17891088650331832No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA204430.16938245046021103No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT186380.15442694867081216No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC166620.13805460986978604No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT147510.12222083484510948No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC142790.11831003326915586No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT140920.11676062671258103No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG139800.11583263989794795No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT135260.11207097906006037No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC131490.10894730915723304No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA122700.10166426978167535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT24500.060.2470551
ACCGTTG30150.049.2667583
GACCGTT35600.041.3295942
GCGATTT46400.035.54641394
CGCAAAA62300.035.086261
CCGTTGA43850.033.6568074
ACCCGGC52700.033.62165
CACCCGG54050.033.0446174
TCACCCG56500.032.613083
TAACCCC69900.030.9240479
GTAGGTC45200.029.0156672
TCACATG79050.027.3541242
CTCGATT40650.026.65413548-49
GTTGACT115550.026.646711
TTAACCC83500.025.4944368
AGTAGGT51800.025.4097581
ATTCCGT43300.025.28940252-53
TTGACTA113600.025.2830642
ATCACAT85450.025.1956541
CGGCCCG72200.024.863368
Produced by FastQC (version 0.11.5)