FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15052963
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT804090.5341739031710899No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG424260.28184484343713595No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA370160.24590507529979314No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG342010.227204438089697No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA324650.2156718248759397No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC320540.2129414654111619No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT241200.1602342342833102No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC236520.15712521182706687No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG227300.15100017185985243No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT207100.13758088689914405No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC191420.12716433302865357No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT189010.12556331932789577No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT181040.12026868065775488No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG174590.11598380996485542No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA172160.11436950984334447No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA168060.11164579358894325No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC166440.11056959350793595No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC162390.10787909330541767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT32200.062.0486071
TCACCCG143100.057.980753
CACCCGG144050.057.0045284
ACCCGGC145450.056.323585
GCGATTT148850.051.49078494
ACCGTTG39250.051.1407363
GACCGTT45450.043.8556062
CGGCCCG187550.043.8243878
CCAGTAG99150.041.193975
TAGCATA102450.040.4570279
GTAGCAT104050.039.745118
CGCAAAA69800.039.7370451
CTCACCC212350.039.0959362
CCCGGCC237800.035.079566
CAGTAGC118050.034.9141356
CCGGCCC240300.034.8498887
CGGACAC136250.034.71422212-13
CTCGATT148700.031.68407648-49
GACACGG149950.031.66795314-15
CGGACAG149500.031.63759418-19
Produced by FastQC (version 0.11.5)