FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15071274
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT800760.531315401737106No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG421910.2799431554359638No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA369910.2454404319104012No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG342640.2273464074768994No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA328400.2178979693422069No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC319440.2119528846731869No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT239640.15900447433972736No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC239630.15899783920058783No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG227670.15106221278970844No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT206830.13723458282292525No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC194450.12902028056818554No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT190660.1265055628343032No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT180730.11991686966874864No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG176580.11716328692584317No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA173130.11487416392270487No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC169400.11239925702365972No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA167080.11085990474328845No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC160580.10654706430259313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT30650.064.251981
TCACCCG143100.055.243993
CACCCGG144300.054.585894
ACCGTTG37350.052.977343
ACCCGGC148800.052.808255
GCGATTT149950.048.91880494
GACCGTT44000.044.9709932
CCAGTAG95750.043.1928945
CGGCCCG189900.041.571998
TAGCATA103100.040.427939
GTAGCAT104650.039.739338
CGCAAAA62750.039.173371
CTCACCC213500.037.116132
CAGTAGC115150.036.198026
CGGACAC137650.034.1205412-13
CCCGGCC241150.032.9130066
CCGGCCC244500.032.750137
CGGACAG149650.031.52555518-19
CTCGATT148050.031.42962348-49
GACACGG151000.031.1193314-15
Produced by FastQC (version 0.11.5)