FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004828903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6543252
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA1967053.006226873120583No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT887961.3570622069882072No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG877211.3406330674716487No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG661161.0104455704900255No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG641030.9796810515627398No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG332330.5078972963291036No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC281190.42974044099172704No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC265640.40597549964451923No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG257100.3929238855541557No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA246500.376723989844805No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA232180.3548388477167011No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC207830.3176249363466362No Hit
CGCAAAACTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT206100.3149809911035063No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG199940.3055667120875063No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG196750.3006914604542206No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG187110.28595872511099985No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA175900.26882657125233755No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA166590.2545981722849739No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG164660.2516485686322336No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG160120.24471012273407777No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT159510.2437778645847661No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT150390.22983984110653233No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG148900.22756268595493495No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT147800.22588156470207782No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC141150.21571842258253238No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT138570.21177542909855832No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC137280.20980393235657133No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT136950.20929959598071415No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT132660.20274322309457132No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA131910.20159700405853234No Hit
CCATCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC126120.1927481931003116No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC115690.17680810703912977No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAG114950.17567717092357135No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG109580.16747024262553237No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC106380.16257970807176614No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAA103770.15859086582635057No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT103670.15843803662154538No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT102670.1569097445734934No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC101200.15466315526285707No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT99830.1525693951570259No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA94890.14501963243964927No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC92130.14080154638702588No Hit
CCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGC87550.13380196880694797No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACC86120.1316165111782337No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT85920.1313108527686233No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG85750.13105104312045446No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGG84280.12880445380981811No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT83030.12689408874975316No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC82250.12570202095227268No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACC80120.12244675888992201No Hit
GCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGT77410.11830508743970124No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA76510.11692962459645448No Hit
GCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAAC76380.11673094663020774No Hit
CGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTC75840.11590566892425967No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC75360.11517208874119475No Hit
CCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCC72720.1111373977343376No Hit
GCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAAC71170.1087685450598571No Hit
GCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGT70750.10812666239967528No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT67790.1036029179374415No Hit
AGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAA66980.10236500137851942No Hit
CCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAG66370.10143274322920773No Hit
CCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACC66260.10126463110392203No Hit
CGTTAAGAGGTAAACGGGTGGGGTCCGCGCAGTCCGCCCGGAGGATTCAA66070.10097425561479216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)