FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12081604
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA256520.21232280084664254No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG205690.1702505726888582No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA175390.14517112131799717No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG133400.1104158024050449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA55750.038.0158654
CCCGTAT61100.036.6482931
TATCGAA59950.035.430925
CGTATCG73600.031.2861023
CCGTATC78550.029.3743672
ATCGAAG92000.024.8245646
TCGAAGG106150.021.7810257
AACGGTT79950.021.689086
CTTAACG81250.021.4577273
CGAAGGC101800.020.9575868
TTAACGG83700.020.8296364
ATCGCGT7500.020.68933794
GTCCGTT8900.020.6574121
TGCACGG64650.020.5707979
TAACGGT86850.020.507025
AGTTTAC169150.019.23658494
GCTACCT78350.018.5316051
TACCTTT73050.018.3340263
CTACCTT83500.017.7278982
TTGCACG77650.017.489998
Produced by FastQC (version 0.11.5)