FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12084905
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAAACTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT513380.42481095217546183No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC357600.295906339354757No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC344370.28495879777292416No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA282340.23363030160352938No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG280150.23181812351855477No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG271180.2243956406773574No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA202850.16785402946899458No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA154160.1275640975249702No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA143340.118610779315187No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC141310.11693099780263064No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT140260.11606214529613597No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG140060.11589664958061316No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC139550.11547463550603004No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG135800.11237159083997764No Hit
CGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATC134720.11147791397615454No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT133760.11068353454164513No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC132640.10975675853471749No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT131790.1090534017437456No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG130770.10820937359457936No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA128550.10637237115227634No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT123710.1023673748366247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAA85900.060.140581
CGACCGT23100.057.792231
GACCGTT28300.046.6740842
ACCGTTG29200.045.2345663
TAACCCC127500.041.279499
GCAAAAC140200.037.8196072
CTAACCC140200.037.7423978
CCGTTGA36550.036.649234
ATCACAT85300.033.1198651
CGCAAGT44950.032.627821
TCACATG91450.031.6630632
CAAAACT169750.031.3737953
GTTGACT105350.029.1814141
ATCGACC102200.028.28767242-43
ACATGCC127600.027.2238582
TTCGGCT105300.026.9102284-85
CGAGCCG100250.026.90474394
CCGCATG106300.026.78376670-71
TTGACTA111400.026.4146612
CCCAAAC163850.026.307891
Produced by FastQC (version 0.11.5)