FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13541348
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAAACTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT1033800.76343950395485No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA650440.4803362264968008No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC548790.4052698446269899No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC482010.35595422257813625No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG467410.3451724303961467No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG428820.31667452900553184No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA413470.3053388776361113No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG310540.22932724275308486No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG288470.21302901306428282No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC267800.19776465385868527No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA257980.19051279089792242No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG244290.18040301453001575No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT234780.17338008003339106No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT232410.17162988500110918No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA230940.17054432099374447No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC227960.1683436538223521No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC218970.1617047283623462No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT214300.1582560318219427No Hit
CGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATC212730.1570966199229205No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG210180.1552134986856552No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG201570.14885519521394766No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT181770.13423331266577007No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA179220.13235019142850477No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA178910.13212126296436663No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT176700.13048922455873668No Hit
CCATCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC173730.12829594217651005No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC167400.12362137063459265No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG164980.12183425165648205No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA162050.1196705084309184No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT157010.11594857469138227No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT155140.11456761911738773No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC151660.11199771248770801No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG141210.10428060780950316No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC140240.10356428326042577No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG135890.10035189997332614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAA134650.075.187751
CGACCGT41400.064.9386751
ACCGTTG47750.056.400243
GACCGTT47850.056.2832032
GCAAAAC197150.052.5675772
TAACCCC206550.049.6423459
CTAACCC227100.045.3778848
CCGTTGA60500.044.744824
CAAAACT242800.042.5092433
ATCACAT104350.040.492541
CGCAAGT56500.038.2663571
TCACATG117800.036.6670842
TGCAACG16800.033.5721931
GCGATTT31400.033.07414294
GTCGTAA7750.032.1394655
TAGCCTT90100.032.081163
CTCCTTG305200.032.02615494
GTTGACT141900.031.7315221
CTAGCCT93350.030.9143562
ATCGACC180800.030.22132742-43
Produced by FastQC (version 0.11.5)