FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13545936
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA519100.3832145670849176No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA478640.3533458300703621No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG311560.23000256313037357No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC238690.17620783089481598No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC211670.1562608888747149No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT197890.1460880960902222No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG195140.14405796690608902No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG183830.13570859924334502No Hit
GTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGA179400.13243824568490506No Hit
CTGCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATT150810.11133228445786249No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG147710.10904377519574875No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC146250.10796596115617259No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG143030.10558886443875122No Hit
AACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT137580.10156551751019642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA79550.041.7051544
CCCGTAT80700.041.416551
TATCGAA83850.039.5103845
CGTATCG109200.033.264583
CCGTATC110050.032.751452
ATCGAAG114850.029.8687526
CCCGTCG23900.029.783811
TCGAAGG129850.026.7802737
CGCGTAG4600.025.620251
CGAAGGC133300.025.3115948
TAGGTAT69200.025.00305294
CTTAACG222850.023.132323
TTAACGG224650.022.884224
TAACGGT226600.022.7495045
AACGGTT232450.022.1365416
CCGGTAA12150.022.1156541
GAAGGCC153300.022.0706719
TCTTAAC235500.021.9695722
CGCTATA2901.1823431E-1021.1322881
CTCTTAA253650.021.1127431
Produced by FastQC (version 0.11.5)