FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004828924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13545936
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAAACTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT1035150.7641775363474329No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA656230.4844478816377104No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC549970.40600368996280506No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC478810.3534713289653812No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG465850.3439038837921573No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG431570.31859740072594467No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA420890.3107131172035657No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG299720.22126193420668752No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG290150.21419708464590412No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC267590.1975426430480699No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA259290.19141534405595892No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG249960.18452766940579077No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT237450.17529242718997048No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT233040.17203683820741514No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA230640.17026508910126253No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC228510.16869266176955214No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC223010.16463240340128582No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG213950.15794405052555985No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT212690.15701388224482973No Hit
CGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATC208420.1538616452934666No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG202040.1491517455862777No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT182810.1349556058732302No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA179700.13265971432317414No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT178640.13187719180129007No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA177890.13132352020561738No Hit
CCATCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC174340.12870280798610004No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC169130.12485663596816048No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG166740.12309226914995021No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA161640.11932730229937598No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT157730.11644082771393575No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT156310.1153925428261288No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC154430.11400467269297597No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC140120.10344061864754123No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG140110.10343323635959893No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG137400.10143263632723498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)