FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14080041
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA203680.1446586696729079No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG153400.10894854638562486No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG140950.10010624258835608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA44350.037.1916774
CCCGTAT52100.034.837631
TATCGAA52700.031.6555585
CCCGTCG43750.031.465191
ACGACTT37550.027.7464831
CGTATCG82000.024.5853083
CCGTATC84950.024.2294182
TGTTACG17100.021.9849833
TCGACCG42850.021.33133130-31
ACCGCGT8600.021.3107346
TTACGAC17650.021.2998985
TGTCGAG18750.021.052825
CGATCCG42600.021.02331582-83
GTTACGA17900.021.0024154
GTTCGAC43250.020.97154628-29
AGACCGC9200.019.9209024
CCGCGTT10100.019.0763247
CGAAGGC88000.018.9573638
TACGACT20150.018.8904486
CGCGTAT2502.3555913E-718.8575631
Produced by FastQC (version 0.11.5)