FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14048696
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT737520.5249739904685815No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG386840.2753565170746096No Hit
CGCAAAACTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT380070.2705375644828531No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC289310.20593370374019054No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC243180.17309791599163368No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA172510.12279431486025465No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC150560.10717008895345162No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG148460.10567528829721991No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG144530.10287787564055768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG124450.054.8049353
ACCCGGC128450.052.8342175
CGCAAAA76150.051.6666641
CGACCGT19800.051.516861
CACCCGG132450.051.3112344
GCGATTT137400.046.06672394
ACCGTTG23300.043.575763
CGGCCCG161400.042.363848
TAACCCC93500.041.8159
CCAGTAG92950.041.2594075
GACCGTT25150.039.623072
CTAACCC100900.038.8428158
TAGCATA102350.038.1534279
GTAGCAT103650.037.63098
GCCAGTA103400.037.5453034
CAGTAGC104250.036.9191256
CTCACCC188700.036.5682682
CCGGCCC216550.032.2913937
CGGACAC130000.031.05011612-13
CTCGATT130400.030.7126248-49
Produced by FastQC (version 0.11.5)