FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16141674
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAAACTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT931320.5769661808310588No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC652580.4042827280491478No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA558110.34575719965599605No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG556060.3444871950703502No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG525230.325387565131101No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC424990.26328743846518027No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT280150.17355696813106247No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC277140.1716922296906752No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG264610.1639297138574351No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT253240.15688583476534093No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC253140.1568238833221387No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT249540.15459363136685825No Hit
CGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATC247020.1530324549981619No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT239580.14842326762391558No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA231840.14362822592006258No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT227200.14075367895547883No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT222010.13753839905328283No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA208120.12893334359249234No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG207880.12878466012880696No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT205100.12706241000778482No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA199340.12349400687933607No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA192680.11936804076206717No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA187370.11607841912802848No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA180940.11209494133012474No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG176810.10953634672587241No Hit
CCATCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC168460.10436340121848577No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA161870.10028080111145846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAA135800.072.348261
CGACCGT42500.062.051961
ACCGTTG52200.050.5190473
GACCGTT52500.050.142842
TAACCCC204550.048.5679559
GCAAAAC214250.047.2392962
CTAACCC228200.043.6582078
ACCCGGC68900.038.472945
CAAAACT262700.038.1674843
ATCACAT140250.038.0094681
CACCCGG71400.036.9963724
TCACATG146250.036.4178472
GCGATTT65450.035.97164594
TCACCCG77950.035.398493
CGCAAGT67300.034.785311
CCGTTGA80250.033.267824
GTTGACT170100.033.246271
TGCAACG23500.032.6062851
CGGCCCG84000.031.944978
ATCGACC171550.031.23250842-43
Produced by FastQC (version 0.11.5)