FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16149671
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG420880.2606121202097553No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA250860.1553344337479073No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG224010.13870870805974933No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA207260.12833697974404557No Hit
GTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGA174770.10821892284988344No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG171310.10607646434407239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT104850.040.2865831
GTATCGA103000.039.920674
TATCGAA109950.037.65375
CGTATCG142300.031.9006273
CCGTATC145000.031.3066162
ATCGAAG160900.027.8624526
TCGAAGG179750.025.1235777
CGCGTAG4400.024.6431391
CGAAGGC177900.023.3772968
GAAGGCC183350.023.1181249
TGCACGG84050.022.3639899
TAGGTAT79800.021.50750494
TACGACG23950.021.1906959
CGCTAGG6300.020.2043131
CCGGTAA16650.019.8200451
ACGACTT27900.019.6008381
AGTTTAC159100.019.35847594
TTGCACG99750.019.0795948
TACCTTT98150.018.3851813
CGGTAAT16900.018.3520362
Produced by FastQC (version 0.11.5)