FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16149671
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAAACTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT924490.5724512901841777No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC654570.4053147584244905No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG558390.3457593656242285No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA556420.3445395265327696No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG528200.32706548634953614No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC427340.2646122016974835No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT279550.1730995015316411No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC279090.17281466600774714No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG269170.16667212601420797No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT252360.15626324523886587No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT251570.15577407118696102No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC249510.15449850340604462No Hit
CGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATC246530.1526532645773403No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT244250.1512414711110833No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA228730.14163136821796557No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT227480.14085735864216678No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT227440.14083259033574122No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA208630.12918529423912103No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG205790.12742674448290617No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT205110.12700568327367165No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA200660.12425020918382795No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA196400.1216123845495057No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA185550.11489398143157219No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA181910.11264006554684612No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG179310.11103012562918464No Hit
CCATCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC169120.10472039956727291No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT162850.10083796753506619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAA133200.072.018011
CGACCGT45850.063.043181
ACCGTTG53800.053.3775443
GACCGTT54400.052.8755462
TAACCCC201800.048.552099
GCAAAAC210200.046.7992482
CTAACCC226700.043.28168
ACCCGGC64450.040.1049275
CACCCGG66900.038.6372874
CAAAACT254900.038.3708953
CGCAAGT66650.038.291471
ATCACAT145200.037.5809671
TCACATG149150.036.7117462
GCGATTT62550.036.6614994
CCGTTGA81500.035.694654
TCACCCG76450.034.8582233
GTTGACT174750.033.3508451
CGGCCCG80500.032.281498
AACTAAC313050.031.5235616
ATCGACC169700.031.18811842-43
Produced by FastQC (version 0.11.5)