FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16156068
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG415460.2571541540924438No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA251970.15595997739053835No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG220860.1367040544766214No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA207330.1283294920521503No Hit
GTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGA171760.10631299645433531No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG171310.10603446333600478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT107350.040.663431
GTATCGA105900.040.1142274
TATCGAA111050.038.2539145
CGTATCG144350.033.0101243
CCGTATC145400.032.480872
ATCGAAG161550.028.5065786
TCGAAGG178250.026.3367357
CGAAGGC178700.024.2718648
GAAGGCC187750.023.2270479
TGCACGG78100.021.7211179
TAGGTAT77650.021.19264694
CGCTAGT3650.020.664371
AGTTTAC159350.019.56230594
CCGGTAA14100.019.3911651
GTTACGA12700.019.240914
TGTTACG13750.019.138653
AACGGTT100200.019.1345586
ACGGTTA67350.018.80386712-13
TTACGAC12500.018.7968885
CTTAACG103150.018.7239973
Produced by FastQC (version 0.11.5)