FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16156068
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAAACTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT929020.5750285279809418No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC649750.4021708747450184No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG559840.3465199577025796No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA554390.3431466121583544No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG527620.32657698643011407No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC428190.26503354652877176No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC278990.17268434374007338No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT278590.1724367587460018No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG269010.16650709813798753No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC253230.1567398701218638No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT250960.1553348252805076No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT250450.15501915441306635No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT247230.15302609521079014No Hit
CGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATC247140.15297038858712403No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT229860.14227471684323192No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA227800.14099965412376328No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT225670.13968126403033213No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA212620.1316038035987469No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT207670.12853993929711116No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG205970.12748770307230695No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA200240.1239410480322316No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA194210.12020870424660259No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA186640.11552315823379798No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA181660.11244072505760684No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG179070.11083761222099336No Hit
CCATCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC168860.10451800524731636No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA161950.10024097447472986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAA138600.070.341131
CGACCGT42750.060.477021
GACCGTT50450.051.432832
ACCGTTG50450.051.2455253
TAACCCC204350.047.7852179
GCAAAAC216350.046.2574042
CTAACCC226250.043.2226688
ACCCGGC63950.040.0536235
ATCACAT149300.039.293311
CAAAACT262900.038.0304343
CGCAAGT63500.037.9758451
TCACATG154750.037.8486062
CACCCGG70250.036.5295454
GCGATTT65450.035.82537594
TCACCCG74850.035.419443
GTTGACT172550.034.026031
CCGTTGA79550.033.0861174
CGGCCCG80650.031.8138588
TGCAACG20450.031.72121
AACTAAC320400.031.1534526
Produced by FastQC (version 0.11.5)