FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15630915
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA297600.19039192523278387No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG227210.14535937275584954No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA204810.13102879773832818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA61550.037.2579424
CCCGTAT67750.035.6205941
TATCGAA67400.034.4424635
CGTATCG103000.026.4160843
CCGTATC107400.025.2901082
ATCGAAG127550.021.0737926
TGCACGG71100.019.6297159
AGTTTAC190800.019.37068794
TCGAAGG143700.019.0650927
CGAAGGC124150.018.7742278
TCAATCG31550.018.3203128
CCCGTCG23800.018.2201271
ACGGTTA54950.017.53126512-13
ATCGCGT13000.017.36196194
GGTACCG57950.016.583220-21
CCGTTAA58750.016.43774430-31
AACGGTT126150.016.3160116
CTTAACG127500.016.2538223
TCGACCG15350.016.22578630-31
CTTCGCG23500.016.1973616
Produced by FastQC (version 0.11.5)