FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14929067
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG194160.13005501281493345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG54550.035.1479533
GTATCGA54350.034.585584
CCCGTAT59600.033.302691
CCGTATC59900.032.9501272
TATCGAA61950.030.7977775
ATCGAAG72550.027.2048596
TCGAAGG94150.021.0633167
CGGGTAT12550.016.9048841
CGAAGGC118050.016.5202548
TTGCACG30800.016.172978
TAGGTAT62950.014.56490894
CGGTAAT12950.014.5152612
TCCGATT101400.014.1350222
CTCCGAT111600.013.9409761
TACCGCG24950.013.93810722-23
CCCGGTT17950.013.9205011
CCGTTAA24750.013.76576530-31
TCAATCG42300.013.6646788
ACGGTTA25950.013.49129212-13
CGGTCGT32800.013.3242794
Produced by FastQC (version 0.11.5)