FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12833351
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA249270.19423609624641297No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT228210.1778257292269182No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA227960.177630924300286No Hit
CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC218790.1704854795914177No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT216550.16874002744879338No Hit
CTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAG213110.1660595116583346No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA205210.15990367597675775No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT191720.14939200213568538No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC190270.14826213356121873No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG183900.14329850403063082No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA182490.14219980424442533No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC180330.1405166896783233No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA176920.1378595504790604No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC163580.1274647595939673No Hit
CACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGG153320.1194699654049827No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG148250.11551932149288209No Hit
GCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAG146840.1144206217066766No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC144560.11264400077579113No Hit
CACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAA134930.10514011500191961No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT134320.1046647909809371No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA133050.10367518195364563No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC130750.10188297662862957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA34300.035.3420834
CCCGTAT37900.032.334791
TATCGAA38200.031.6108595
TGTCGAG21400.029.4210075
ACGACTT35700.027.7259851
GTTACGA14450.027.6386994
TACGACG71350.026.8678389
TTCGCGC59050.026.8149227
GGTACGG91400.025.98046394
CTACGAC74550.025.714568
TGTTACG15050.025.6002313
GTCGAGG27200.023.6656386
TTACGAC17350.023.2897875
TACGACT17150.023.2874186
GCGCGTT14850.021.847790-91
GCACCCG31650.021.2289668
CCGTATC153850.020.980982
ACCCGGT18350.020.74032210-11
CGTATCG160300.020.6057453
CCGCTTC81450.020.5364533
Produced by FastQC (version 0.11.5)