FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12833351
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1059760.8257858761908716No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG400840.3123424271649704No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA360780.28112688572143No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT200290.15606991502063647No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA188920.14721018695740495No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG181410.1413582469613743No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC174870.1362621500806765No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC165740.12914787416006934No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG162870.12691151360233194No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA161420.1257816450278653No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA150470.11724918924137584No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG147640.11504399747189958No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG147290.11477127057461453No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT138790.10814790306912045No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT138630.10802322791607585No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT137230.10693232032693564No Hit
CGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCG134770.10501543984887503No Hit
CAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGC129630.10101025055731741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG174300.060.435793
CACCCGG178250.058.9073524
ACCCGGC193900.054.101835
CGACCGT15700.053.5951081
CTCACCC225750.046.6839682
CCAGTAG85300.046.228365
ACCGTTG19100.043.8062823
GCGATTT218200.043.78452794
GTAGCAT94350.042.3378338
TAGCATA96700.041.3086179
CAGTAGC101000.039.4581076
CGGCCCG275450.038.234088
GACCGTT22100.037.860612
GCCAGTA113350.034.8730854
CGGACAC174550.034.62296312-13
TGCCAGT116650.034.0100633
CCCGGCC321850.033.3068476
GACACGG183500.033.0365814-15
CCTCACC326900.032.9012831
CGGACAG185550.032.5196218-19
Produced by FastQC (version 0.11.5)