FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12834937
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA250160.19490551453427468No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT225990.17607410149344715No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA223500.17413408417976653No Hit
CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC217770.16966970698804365No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT214290.16695835748940568No Hit
CTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAG212280.1653923194169165No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA207790.1618940552649382No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC191200.14896839774125886No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT190810.14866453960779083No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG185500.14452739425211047No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA184980.14412225007415308No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC184450.14370931466200418No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA175740.13692314968121777No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC163110.1270828208973679No Hit
CACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGG152930.11915134449043265No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG149760.1166815232517308No Hit
GCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAG146870.11442985657039065No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC142760.11122765931768891No Hit
CACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAA136680.10649058892926391No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA134930.10512712294575345No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT133620.10410647126666847No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC132590.10330397414494516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA35600.037.752634
CCCGTAT38200.036.6453441
TATCGAA40350.033.4248545
TGTCGAG21450.029.575885
ACGACTT39300.027.5842361
TACGACG73450.026.8713029
TGTTACG15700.025.7412723
CTACGAC77050.025.5548068
GTTACGA15850.025.4976634
GGTACGG94350.025.41724894
GTCGAGG26150.024.4398426
TTCGCGC60350.024.3723987
GCGCGTT12500.023.69728790-91
CCGTATC153100.022.775082
TTACGAC17700.022.301665
GCACCCG28500.022.259748
CGTATCG160700.022.1366163
TACGACT19050.020.721236
GCCGCAG58700.020.7160641
ACCCGGT17450.020.46677210-11
Produced by FastQC (version 0.11.5)