FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12834937
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1054040.821227248719647No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG402030.31323098820040957No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA361520.28166869849068993No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT200000.1558246838297687No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA189520.14765947039708882No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG182470.1421666502920895No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC172480.13438320733479253No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC165950.1292955314077506No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG163760.12758925111981462No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA160060.1247064944689639No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA151520.11805278046943277No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG149570.11653348980209252No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG148670.11583227872485856No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT138550.10794754972307226No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT138000.1075190318425404No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT136780.10656850127117883No Hit
CGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCG135380.10547772848437044No Hit
CAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGC129330.10076403179851993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG178150.061.5482253
CACCCGG178200.061.2106484
ACCCGGC192600.056.5361065
CGACCGT17250.053.129681
CTCACCC227350.048.2707022
CCAGTAG81750.046.2194185
GCGATTT217600.046.2154794
ACCGTTG19850.045.9333233
TAGCATA91100.042.0919659
GTAGCAT90200.041.7825788
GACCGTT22200.040.8597832
CGGCCCG272700.040.306268
CAGTAGC97750.038.6525846
CGGACAC177500.035.7794212-13
GCCAGTA109350.034.89774
CCCGGCC320250.034.7921836
CCTCACC322100.034.7424431
GACACGG184050.034.59525314-15
CGGACAG187350.033.87308518-19
TGCCAGT116700.032.7420853
Produced by FastQC (version 0.11.5)