FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13681674
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG180540.13195753677510516No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA150990.11035930252394555No Hit
CTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCA141430.10337185347348578No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT141200.1032037453896358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGG5900.027.9658011
GTATCGA41600.023.9483474
CCCGTAT44100.023.8384251
CGTATCG57200.023.0857143
TATCGAA43900.022.8006975
CCGTATC61000.022.0327782
TAGCGTG39350.019.04808618-19
CGGTAAT11350.019.0456142
CCGGTAA11550.018.7753091
TTGCACG53700.018.4646538
CGCTAGT2302.2156346E-618.4470081
GTCCGAT5150.018.307711
CGCTACT4900.017.31761
GTCCGTT8550.017.0925481
CGCCTAC6100.017.002161
TACGACG15550.016.9235179
TTCGCGC19300.016.80067
GCTACCT62450.016.5319061
ACGGTTA42500.016.419912-13
ATCGAAG65850.016.2709186
Produced by FastQC (version 0.11.5)