FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13701110
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG180230.1315440865740075No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA150650.10995459491968168No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT145460.1061665806639024No Hit
CTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCA141430.10322521313966533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAG3800.023.5711361
CGCTAGG5700.023.1576061
CGTATCG57700.022.5596223
GTATCGA41950.022.1799774
CCCGTAT45000.021.8949661
CCGTATC60650.021.5398082
TATCGAA46050.020.8174925
TTGCACG53900.019.8780578
TAGCGTG37200.019.39069218-19
CCGGTAA10800.019.206111
CGGTAAT10750.017.9226932
GCTACCT61700.017.7261051
CGCGTTT3802.3283064E-1017.3682061
GTCCGTT8150.017.3529821
TTCGCGC16600.017.2683017
GTCGCGA2504.9084592E-616.9712161
CGCTACT5000.016.9712161
GCCGTAT24700.016.7956281
CGCGTAA20000.016.7355041
TGCACGG64450.016.4054249
Produced by FastQC (version 0.11.5)