FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13701110
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC257660.18805775590444862No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG187410.13678453789510484No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA169390.12363231884132016No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC165340.12067635395964268No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG141260.10310113560142208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT16350.048.8771
ACCGTTG21200.037.6939963
GACCGTT25300.031.5860582
CACCCGG41250.029.9663494
ACCCGGC43150.027.9922925
GCGATTT39800.027.39114694
CTCGATT31950.024.20167448-49
GTTGACT74150.023.3931431
CCGTTGA36250.022.6898734
CGGCCCG56650.022.4801128
CGGACAC35600.021.71338712-13
ATTCCGT35550.021.5481352-53
TCACCCG60100.021.4306433
GTAGGTC31050.021.1950022
TTCTCGA37150.020.81545846-47
CCGTATG44400.020.5362324
ACCGTAT48400.019.3271053
CGTATGG47750.018.9963475
CCGTCAT71800.018.5937611
GACACGG42400.018.5634314-15
Produced by FastQC (version 0.11.5)