FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15304921
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA234080.15294427197631402No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC179690.11740668246507119No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT167050.10914790086142881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA47150.031.6887044
CCCGTCG32900.031.2336181
CCCGTAT52600.029.034941
TATCGAA52100.028.5877885
CGTATCG69900.026.349233
CCGTATC72900.024.9426422
CGGTAAT13700.024.6928352
GCGCGTT9150.022.09805990-91
CCGGTAA17300.020.43511
ACGACTT30400.020.1572571
ATCGAAG80350.019.706216
CTTCGCG23950.018.8332276
CGGCATG49750.018.6995496
CGTACGC42150.017.8353425
CCGTACG43850.017.143894
TCCGTAC46750.016.9849433
GTACGCC45050.016.8958196
CGCGTAA37150.016.8754161
TCGACCG26100.016.65176430-31
GTTACGA12500.016.5387134
Produced by FastQC (version 0.11.5)