FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15304921
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC335320.21909293095991803No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT265890.17372843675573366No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC254430.16624064900433003No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT224000.14635815500125743No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG215140.14056916726326127No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG210530.13755706416256575No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG207120.13532902260651983No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA183730.12004635633205818No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACA176200.11512637013938196No Hit
AGGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACAC168420.11004303779157043No Hit
CGTGCACCCACTGCCTCTTCCCTTCTCGCTTGGGAACTCTAGTCTCGCCT162450.10614233160693871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT20450.050.5743751
ACCGTTG24550.041.1670533
CGTGCAC40900.040.5744441
CACCCGG70350.040.554784
ACCCGGC70200.040.4366045
GCGATTT73150.035.59009694
CCAGTAG66250.032.703175
TCACCCG89050.032.464153
CGGCCCG88500.032.0196238
GACCGTT31750.031.8334062
TAGCATA72050.031.242259
CGCAAAA69500.030.6418231
GTGCACC57950.028.3924562
GTAGCAT80700.027.2530148
CTCGATT62400.025.87076248-49
CGGACAC65350.025.13247512-13
ATTCCGT65200.024.61397652-53
TGCACCC67550.024.4951523
CAGTAGC89200.024.3404066
GTAGGTC40600.024.0838382
Produced by FastQC (version 0.11.5)