FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004828983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004828983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15319322
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA235750.15389062257454997No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC180370.11774019764060055No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT168280.10984820346487918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA48450.028.0300334
CCCGTCG28400.027.8884661
CCCGTAT51700.026.3536591
TATCGAA52600.026.1758885
CGTATCG70350.026.050743
CCGTATC74100.025.0494632
CGGTAAT14350.024.8874992
CCGGTAA16250.022.9196191
GCGCGTT9450.021.89297990-91
ATCGAAG79250.018.7966126
CGTACGC42800.018.115895
GGCGCGT11750.017.60754490-91
CTTCGCG25100.017.598426
CGCAAGT39250.017.53951336-37
CCGTACG44100.017.4753064
TCGACCG21900.017.38088230-31
ACCGCGT8150.017.2974956
GCCGTAT29850.017.215351
GTACGCC45750.016.9477636
CGCGTTT4205.2750693E-1116.8374231
Produced by FastQC (version 0.11.5)