FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10161700
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA210230.20688467480834902No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG200620.1974275957762973No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC142810.14053750848775304No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA135410.13325526240688074No Hit
CAAGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGA128860.12680949053800053No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC125560.12356200242085477No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG122810.12085576232323332No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC122100.12015706033439287No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA119570.11766731944458113No Hit
GGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGC117220.11535471427025007No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG116090.1142426956119547No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG106250.104559276499011No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT105190.10351614395229146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT11450.042.6984861
GTAGGTC27750.033.2028662
ACCGTTG16050.030.4591163
AGTAGGT30450.030.4133011
GTCGTAA8250.028.484095
CTTAGCG12000.027.4128328
GCGATTT18850.026.17632794
TCGTAAG9400.025.9976446
GCGGGTC42450.025.9131762
CATTCTT62450.025.366263
TAACCCC40900.025.047729
TAGGTCT38500.024.663213
TCACATG53150.024.4996012
GACCGTT19950.024.2703952
ATTCTTG68350.023.4497644
CCGTTGA20450.023.4438824
CGAGCCG53100.023.09812494
ATCACAT57700.022.4048231
TACTATA29750.021.6449344
GTCTAAC17500.021.486944
Produced by FastQC (version 0.11.5)