FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10184278
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA208560.20478624012423854No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG202940.1992679304315927No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC142220.13964662001567515No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA134880.13243943262350064No Hit
CAAGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGA129170.12683275142332132No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC123530.12129480361789025No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC123520.12128498456149762No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG121640.11943900195968728No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA119890.11772066709098082No Hit
GGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGC117820.1156881224177109No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG113500.11144629005610412No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT106200.10427837888950008No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG106140.10421946455114442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT11350.039.7525831
GTAGGTC28900.032.687992
ACCGTTG15150.029.4709783
CTTAGCG13150.028.5884238
GCGGGTC45850.027.9842262
AGTAGGT34350.027.7753331
TAGGTCT38350.025.3681343
CATTCTT64400.025.2507213
TCAGACG38250.023.9603482
ATTCTTG68650.023.8226624
GTTGACT66050.023.4106271
GTCGTAA8550.023.085265
CCGTTGA20000.023.0275464
GACCGTT20200.022.8015652
GCGATTT20900.022.48420594
TCACATG52050.022.3031482
GTCTAAC18150.022.2676014
CCTAGAG62650.022.2055021
CGCGGGT58900.022.0233691
TAACCCC41650.021.77549
Produced by FastQC (version 0.11.5)