FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10951586
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT331260.3024767371593484No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG286190.26132288053985975No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG245980.22460673732553443No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC245200.22389451171729832No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA181000.16527286550094206No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA172990.15795885637020976No Hit
CAAGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGA163400.14920213382792227No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA142610.13021858203916767No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC138160.12615524363320527No Hit
GGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGC133540.12193667656903759No Hit
CCCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTAC121690.1111163259823737No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC119060.10871484732896221No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT117120.10694341440591344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG70550.047.971063
CACCCGG70600.047.3986054
ACCCGGC74500.045.1686555
CCAGTAG63350.044.7353745
TAGCATA64950.043.9879159
GTAGCAT70400.041.1848378
CAGTAGC73800.038.5242166
GCGATTT81450.038.42114694
CGACCGT10550.038.3187831
CATTCTT74050.032.7543143
GCCAGTA88350.032.396724
AGTAGCA90000.032.007287
CGGCCCG110100.030.8585728
ACCGTTG14050.029.7753943
CGGACAC64650.029.76353312-13
GTAGGTC28300.029.0672152
GTCTAAC36700.028.9422764
CTCGATT66850.028.65450348-49
TGCCAGT102350.028.336173
ATTCCGT70950.026.5960152-53
Produced by FastQC (version 0.11.5)