FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10879617
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA407880.37490290329154047No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA322160.29611336502011054No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC241340.2218276617641963No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT235900.21682748574697067No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA229850.21126662822781353No Hit
GTGATGTTATAAGCCTAAGGCAGCTTGACTTGCAGCAACAAGTTTTTTAC227010.20865624221882076No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC208870.19198286116138097No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA206790.19007102915479468No Hit
CTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCA192860.17726726961068573No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC182930.16814011007924268No Hit
CTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCAT175310.16113618705511415No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA164190.150915239019903No Hit
CTTCATTTTCTTTCTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATG155030.1424958249908981No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC152240.13993139648206368No Hit
GTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATC143720.1321002384550853No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG138930.12769750993991794No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT128070.11771554090553003No Hit
GGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTG113110.10396505685815961No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT111810.1027701618540432No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG111490.10247603385302993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA18900.034.80964
CCCGTAT25150.029.052661
TATCGAA23950.028.2546425
CCCGTCG26450.027.268291
ACGACTT20850.026.9050051
TGTCGAG14600.026.3933097
GTCGAGG16250.023.7133738
CGCTAAT1802.1030246E-723.570141
GTCGGTA8150.022.5579281
GAACGGG27650.022.0943439
GTTCGAC17300.021.4595628-29
AGGAACG29000.021.2278567
TCGACCG17900.021.00253930-31
TTACGAC6900.020.4317255
CGACCGT18550.020.39317332-33
GTTACGA7150.020.3745734
CGCTAGG4650.020.275391
CGTATCG50250.020.199963
AAGTTCG19400.019.50001726-27
CCGTATC52950.019.4361842
Produced by FastQC (version 0.11.5)