FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10863425
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA407740.3753328255131324No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA319770.29435468095927386No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC238300.21935991641678382No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA233040.21451798120758417No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT232460.21398407960656973No Hit
GTGATGTTATAAGCCTAAGGCAGCTTGACTTGCAGCAACAAGTTTTTTAC226670.20865426879644308No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC208790.1921953711651712No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA205970.18959950476023904No Hit
CTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCA193270.1779089007380269No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC181360.16694550751719647No Hit
CTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCAT174020.1601888907043589No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA160860.14807484748134223No Hit
CTTCATTTTCTTTCTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATG151570.1395232166650941No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC151160.13914580346437702No Hit
GTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATC143200.1318184642504551No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG141630.13037324784770918No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT128580.11836046182488488No Hit
GGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTG113410.10439617339835273No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT112190.10327313899621895No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG111090.10226056699429507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA19400.032.216284
CCCGTCG25100.029.1147461
CGGTAAT7950.027.7815342
TATCGAA22650.027.5936345
CCCGTAT23750.026.9979211
CCGGTAA8250.026.2880821
TGTCGAG16750.024.6884235
GTCGGTA7800.024.1780011
GTCGAGG18550.022.292786
GAACGGG28600.020.8671519
ACGACTT21400.020.7094761
AGGAACG27800.020.6224617
TCGGTAT7400.019.6859072
GTTACGA7200.019.6446271
ATCGCGT11150.019.39743694
CGTATCG53850.019.1982843
CGGCATG36650.018.9763746
CGACCGT17050.018.8795532-33
CGTCGAC2251.8512201E-618.7968653
ACGCTCA14150.018.5976073
Produced by FastQC (version 0.11.5)