FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7311023
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA672060.919242081443322No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG440590.602637961883036No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG301110.411857547158585No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT222360.30414348306659683No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG198130.27100174626724605No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA132210.1808365258870065No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC128950.17637750558300802No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC125640.17185009539704635No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG121650.1663925828163856No Hit
GCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGT108430.14831029802532422No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA108300.14813248433221998No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG108150.1479273146863305No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA103290.14127981815951063No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG102510.14021293600088525No Hit
AAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG100550.13753205262792909No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG94610.12940733465070484No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC93890.12842252035043522No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC91680.12539968756766323No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG81850.11195423677370457No Hit
GGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAG79310.10848003076997569No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC78680.10761831825723978No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT77400.10586753727898271No Hit
CTCAGTAACGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCC76250.10429456999382987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTG158050.037.0013771
TCAGACG57100.032.2725332
GCCGTAT8650.030.69904346-47
GCGATTT22250.029.3575294
TCACCCG26450.028.0790143
GGTGTGA205500.027.9767822
CGACCGT5300.027.4965291
GCGAACG3900.026.516033
CGTATCA10550.026.28169648-49
TCGTAAG6800.026.2628546
CACCCGG27500.026.1506444
CGGTGTT10250.026.142251
GTCTAAC34600.025.6749384
TAACACG37250.024.4749557
CTTAGCG8900.024.2889278
CTCGATT18250.023.56199648-49
CAGACGT78000.023.442583
AACACGT38800.023.3757788
GTGTGAG284700.023.282181
CATCTAA269750.023.08282594
Produced by FastQC (version 0.11.5)