FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7308881
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA452070.6185214946036198No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA292260.39986969277513207No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC245240.3355369994394491No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC188170.25745391120747485No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC164720.22536965644946197No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT151120.20676215688831162No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG142580.19507774172270695No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA142500.19496828584293546No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC128990.17648392414652803No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA121410.16611297953818102No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC111970.15319718572514726No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC109560.14989982734703164No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG106640.1459046877353729No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT102030.1395972926635418No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT98730.13508223762296853No Hit
AACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT94070.12870643262628026No Hit
GGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCC89570.12254953938913495No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC89310.12219380777987766No Hit
GCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCC86270.11803448434856169No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT80470.11009893306512994No Hit
ACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTT79630.10894964632752947No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG78660.10762249378530038No Hit
GTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGC75910.10385994791815602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG12300.033.23904444-45
GTCGGTA7150.030.9847051
TATGCCG14750.027.39938548-49
CGGTAAT6550.027.2631912
TATCTCG15450.025.245540-41
TCGGTAT8200.025.2158262
AGGAACG25950.024.8094777
GTCGGGA28650.024.3496461
GAACGGG26100.024.3067919
CGTATGC16800.024.19586846-47
CCGGTAA7750.023.1119961
GTATCGA6450.022.5858484
CTCGTAT18400.021.83648344-45
GCCGTCT21250.020.45583350-51
GTATGCC20650.019.91234446-47
CGTATCG20900.019.112043
GTCGAGG11600.019.040326
AACCCGC7250.018.854521
TGTCGAG10600.018.6199195
CCCGTCG14550.018.1418081
Produced by FastQC (version 0.11.5)