FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13204107
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA608590.46090962455848017No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA363760.2754900426056832No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC271140.20534520055010158No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC221030.16739488706051836No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC205590.15570155558418303No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT194220.1470905984024516No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG165740.12552155174143925No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA161080.12199234677513594No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC158860.12031105170535199No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA151160.11447953276961478No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT148560.11251044845365157No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC137180.10389191787070493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAT9950.025.5036832
TCGTATG13850.025.1081244-45
GTCGGGA46700.023.3202951
GTATCGA12000.022.7132364
GAACGGG42600.022.5036419
AGGAACG43050.022.486737
GTCGGTA10100.020.0717891
AGTTTAC418650.019.16050394
TGTCGAG19450.019.087097
CCGGTAA13100.018.714191
GTCCGAT12800.018.4161571
TATGCCG16900.018.35225748-49
CGATTGG9450.017.404784
CCCGTCG25900.017.29271
CGCGTAA17750.015.93646051
CGTATCG44400.015.7701413
GTCGAGG23300.015.5298418
TCGGGAG70550.014.9871052
GTCGTAT5055.4569682E-1114.9371441
GTTACGA6350.014.8489321
Produced by FastQC (version 0.11.5)