FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13924598
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA797480.572713122490143No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA525880.3776626082849932No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC382030.27435621480778116No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT295830.2124513756160142No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC293670.21090016386828545No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA288660.20730221439785912No Hit
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCC253200.18183648820597909No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG246590.1770894929964944No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT244770.17578245346831556No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC243460.17484167226946157No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC241600.1735059065978063No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA213600.15339760616428566No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC195850.14065037999660746No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC190850.1370596120620502No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT189260.135917747858861No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT179080.12860694434410241No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG170650.1225529096064389No Hit
GGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCC165630.11894777860014343No Hit
CCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTT146270.10504432515753775No Hit
AACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT143810.1032776673337356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGGA72400.037.8265651
GTATCGA25800.036.9699334
GAACGGG74050.036.4850659
AGGAACG74250.036.070387
GGAACGG93900.028.5721458
CCCGTAT37400.028.2316171
CGTATCG44000.028.191853
TCGGGAG101200.027.300412
CCGTATC48800.026.3817182
TATCGAA45050.023.154245
CGCGTAA9800.023.0873721
GAGGAAC119550.022.7562836
GTCGGTA11200.020.6223141
AACGGGG69550.020.16598910-11
CGGGAGG139450.019.8458213
GGGTCGG64150.018.750622
GCGTAAC12050.018.716582
AGTTTAC568600.018.3091994
GTCCGAT14750.018.2155461
AGATCCG80000.017.50319524-25
Produced by FastQC (version 0.11.5)