FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13926489
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA799250.5739063162294531No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA529780.38041174627718444No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC381020.27359372487925704No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT294860.21172601364206012No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC294360.21136698560563255No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA290120.20832242785672686No Hit
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCC257260.18472710530270767No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG247900.17800610046078377No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT244490.17555752925234783No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC243060.17453070906816498No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC241400.1733387359872255No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA213230.1531110964148968No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC196330.14097594878364533No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC191890.1377877798201686No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT190950.1371128071116848No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT181890.13060721909161743No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG170790.12263679668292561No Hit
GGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCC165620.11892444678626465No Hit
CCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTT148580.10668877130481343No Hit
AACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT146630.10528856196274596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGGA70100.034.431491
GAACGGG69500.033.9435969
AGGAACG70250.033.7157
GTATCGA25850.030.90494
GGAACGG86750.027.1940088
GTCGGTA10400.025.83721
CGTATCG45900.024.981363
TCGGGAG98450.024.6782192
CCCGTAT38350.023.4785821
CCGTATC50400.023.4035722
CGCGTAA9400.022.5677641
GGGTCGG61400.020.664952
GAGGAAC118100.020.4129836
TATCGAA44000.019.6518825
GCGTAAC12200.019.2596842
AACGGGG66650.018.26159110-11
TCGCGAT5500.017.95256294
AGTTTAC561450.017.83767794
GTCCGAT17550.017.7284471
CGGGAGG140250.017.222623
Produced by FastQC (version 0.11.5)