FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13624537
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG208010.1526730779915677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA57650.037.386674
CGTATCG62900.035.535283
CCCGTAT60950.035.4594081
CCGTATC66650.033.5356752
TATCGAA83300.026.6071785
ATCGAAG89000.025.957966
TCGAAGG104800.022.6714827
CGAAGGC117250.018.982628
CGCGTAA13000.018.070661
TTCGAAA34750.017.161076
CGAAAGG34250.016.8630148
CGTTTCG45050.015.6350723
TAGGTAT44800.015.61687594
CTCGAGT42900.015.551721
TCGACCG12300.015.46195730-31
CGAGTGA38000.015.446493
GCGTAAC14950.015.3905852
GTTCGAC12400.015.14797128-29
CGATCCG12200.014.81820382-83
CGTTTAA16500.014.798625
Produced by FastQC (version 0.11.5)