FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14722897
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG157960.10728866744092551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA54750.031.3319384
CCCGTAT58100.029.7031841
CCCGTCG42750.028.3789731
CGTATCG66400.027.816533
CCGTATC69700.026.8367712
TCGACCG18000.022.45329730-31
TATCGAA81750.021.1562445
GTTCGAC18950.020.83178328-29
CGCGTAA30950.020.8148371
ACGACTT22450.020.5268751
ATCGAAG94100.019.9764986
GCGTAAC34850.019.2847142
CGATCCG20750.018.57121582-83
CGGCATG64350.017.9653436
CGTCGGC67500.017.5458833
TCGAAGG112850.016.907047
AAGTTCG23600.016.82678226-27
CGTAACT40150.016.7389813
CGAAGGC112100.015.7625078
CGACCGT20200.015.35468232-33
Produced by FastQC (version 0.11.5)