FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14861582
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG158570.10669792758267592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA53700.032.441114
CCCGTAT57200.030.717031
CCCGTCG37300.029.7239691
CGTATCG68650.028.1807633
CCGTATC72050.026.524892
TATCGAA78250.022.9231155
ATCGAAG93550.021.1314166
CGCGTAA30100.020.2899271
ACGACTT22800.019.368531
GCGTAAC33250.019.0649342
TCGAAGG111100.017.7932557
TCGACCG19400.017.67004630-31
CGGCATG61450.017.2693826
GTTACGA8300.016.9722424
CGAAGGC108600.016.516618
CGTAACT41250.016.1644343
CGTCGGC67400.015.8844093
CGATCCG21200.015.72590582-83
GTTCGAC21400.015.5797228-29
CGACCGT20950.015.57804532-33
Produced by FastQC (version 0.11.5)