FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14059820
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA372320.26481135604865497No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG180790.1285862834659334No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC155020.11025745706559544No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG153280.10901988787907668No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT152550.10850067781806595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTT45200.026.5047551
CCCGTAT38500.024.1616461
CCCGTCG84500.022.461911
GTATCGA46600.019.6494244
TATCGAA46650.019.5275655
AGTTTAC268150.018.8417794
CGACTTT77350.018.0905782
ACTAAAC36450.017.77742294
CTAGATA59900.017.32405716-17
GTTACGA16650.017.2034974
GAACTCT315450.017.147899
CCGTCGG108900.017.118242
AAGTTCG62500.016.4921226-27
TTACTTC89350.016.3438598
TTGAACT339050.016.286677
CATGTAT110700.016.1185059
CCTCTTG306950.015.9710683
CTTGAAC347500.015.4179746
TGTTACG18650.015.3586183
TCTTGAA341050.015.296575
Produced by FastQC (version 0.11.5)