FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12460883
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGCTGC220370.17684942551823976No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT200470.16087944971475937No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG185520.14888190507847637No Hit
GGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGC160940.12915617617146394No Hit
CAAGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGA157080.12605848237239686No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG149640.12008779795139718No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA149190.11972666784528833No Hit
CGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAGCTGCTCGCAG125630.1008195005121226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT11400.040.3764151
TCACCCG56500.038.821733
GCGATTT53450.037.38051294
ACCCGGC57300.037.3783575
CACCCGG61750.034.9127434
CGCCGTA19100.030.14783746-47
GCCGTAT20650.028.90926746-47
GACCGTT16650.027.6451132
CGACCTA12450.026.7853454
ACCGTTG17500.026.570743
CTCGATT47050.026.27483448-49
CCAGTAG54300.025.9496445
TAGCATA57100.025.2532629
CGGCCCG86900.025.1329338
GTAGCAT56300.025.1112378
ATTCCGT48250.024.9408352-53
CCGTATC24050.024.52858548-49
ACGTGCG21100.024.4861328
AGAGCGT67800.023.6922558
GTCTAAC22700.023.3807851
Produced by FastQC (version 0.11.5)