FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12526522
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA176050.1405418040218985No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT150930.12048835263291759TruSeq Adapter, Index 20 (97% over 44bp)
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT140290.11199437481529191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23200.034.9311348-49
TCGTATG24650.032.97105844-45
CCCGTAT22700.030.8388331
GTATCGA20000.029.8181464
CGTATGC31450.026.14140546-47
TATCTCG28800.025.9346140-41
TATCGAA24800.024.9933225
CTCGTAT32650.024.24490444-45
CCCGTCG23750.023.145141
TGTCGAG15150.021.3864255
GTATGCC39500.020.81385446-47
GCCGTCT40400.020.326850-51
GTCACGT42100.019.79824328-29
CGGAAGA84050.019.7775484
GATCGGA84250.019.5737821
TCGGAAG84850.019.5357363
ACGTGGC45600.018.58724232-33
TCGACCG15500.018.4802830-31
CGTATCG47150.018.1257743
ACCCGTT40700.017.7677142
Produced by FastQC (version 0.11.5)