FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13703826
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA366680.267574909372025No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT204770.1494254232358175No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT191690.13988064355166216No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA169910.12398727187575206No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC151190.11032685324521779No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG144450.10540851875965151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA38000.042.17294
CCCGTAT42100.039.9851071
TATCGAA45800.035.1957135
CCCGTCG31050.031.1962851
TTCGCGC25400.028.30777
ACGACTT27750.025.7559661
CGTATCG79700.024.2941423
ATCGAAG86050.024.193826
CTTCGCG30350.023.8458966
CCGTATC82600.023.6687012
TGTCGAG15600.023.1967545
TCGACCG28750.022.23083930-31
GTTCGAC29900.021.45466828-29
CGGCATG62600.021.0206623
TCGAAGG103150.020.6839497
CGATCCG31600.020.52283182-83
AGTTTAC236550.019.80737794
AAGTTCG33900.018.92324626-27
GTCGAGG19750.018.560056
GTTACGA8800.018.157624
Produced by FastQC (version 0.11.5)