FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13722852
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA370750.27016978686354703No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT208500.1519363467594054No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT193210.140794347997049No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA173990.12678851305836425No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC149960.10927757582753206No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG145950.10635544273158379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA37350.036.6176454
CCCGTAT42200.034.2129861
TATCGAA44550.031.5436175
CCCGTCG32650.027.511561
TTCGCGC24500.025.7028267
ACGACTT26750.024.7890781
CTTCGCG27350.023.0253076
GTTACGA9200.021.9668944
CGTATCG79250.021.8834173
CCGTATC80950.021.5401272
CGCGTAT1754.42045E-621.4989361
ATCGAAG84450.020.5901746
TGTCGAG17650.020.2375015
TTACGAC10250.020.1751525
TCGACCG32050.020.16180830-31
CCGGTAA12350.019.8016531
GTTCGAC32550.019.77991128-29
AGTTTAC238050.019.24768894
CGATCCG33350.019.16395882-83
CGGCATG64750.018.073723
Produced by FastQC (version 0.11.5)