FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13935533
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA381070.27345204521420174No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT208580.1496749352895221No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT198130.14217611913372813No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA178250.12791042868615074No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC158220.11353709972915998No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG146460.10509824059115644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA39200.038.333314
CCCGTAT44800.036.809221
TATCGAA46700.033.282845
TTCGCGC26600.027.0086887
CCCGTCG33750.026.8664951
ACGACTT24150.026.4575271
CTTCGCG30000.024.574056
CGTATCG79250.023.9383663
CCGTATC82850.023.2946132
ATCGAAG85950.022.8364586
TCGACCG30550.021.9039830-31
GTTCGAC31650.020.99437328-29
GTTACGA7950.020.6734664
CGATCCG33650.019.74528982-83
AGTTTAC238100.019.5238294
AAGTTCG35650.018.7043926-27
TCGAAGG104000.018.6922117
CCGGTAA10850.018.6194231
TGTCGAG18550.018.4794145
CGGCATG65200.018.1495293
Produced by FastQC (version 0.11.5)