FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14612077
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT923710.6321551686320843No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA901990.61729075202656No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG774710.5301847232258631No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT567300.3882404944895924No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC560710.3837305264679347No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA454150.3108045488673513No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG434430.29730886307264875No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC407970.2792005544454768No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG336680.2304121446937352No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT322510.220714686899063No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC264540.1810420243473943No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA257160.17599140765546198No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG257080.1759366584230291No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA256000.17519754378518537No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG248080.1697773697743312No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG246860.1689424439797299No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG235600.1612364895148034No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT226170.1547829237417788No Hit
CAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCCAAGGCAA225730.15448180296339803No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC219370.15012923898498481No Hit
CGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTG215000.14713856216333926No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT214850.14703590735252764No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG212150.14518812075791826No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA202600.13865243113624434No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT192660.13184983900646022No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT182240.12471875148207882No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA179670.12295993239017286No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACA177610.12155013965502645No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT176340.12068099559015463No Hit
AGGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACAC166610.11402212019550678No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA164960.11289291727657881No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT164690.11270813861711788No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG161960.11083982106034618No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG160980.1101691429630435No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC155410.1063572276549049No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA155140.10617244899544397No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC153400.10498165319002904No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATG152650.10446837913597089No Hit
CAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCCAA148900.10190200886568009No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG147070.10064962017377815No Hit
CTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAA147000.1006017145953994No Hit
AGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCC146290.10011581515755767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG155600.060.456033
CACCCGG158550.058.887064
CCAGTAG142650.052.742475
TAGCATA152350.049.1999479
TGCCAGT154650.048.740883
CAGTAGC157750.047.7534456
CTCACCC201100.046.614042
GTAGCAT163650.045.773698
CGACCGT7500.045.7119641
ACCCGGC204750.045.622665
GCCAGTA180150.041.8418164
CCTCACC237700.040.4245031
TCAGACG107650.038.8715552
CTGCCAG197500.038.213522
GCGATTT223250.037.92712894
CGGTGTT11650.037.490851
ACCGTTG9200.036.7547043
GTCTAAC47300.036.737441
CGGACAC157300.036.3976812-13
CCTGCCA214550.035.5926441
Produced by FastQC (version 0.11.5)