FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004829172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14582449
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA818370.5612020312911775No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG416120.28535673260369365No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC414880.28450639532495536No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA378240.2593803002499786No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA372670.25556064005435575No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC314190.21545763677966573No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT307660.2109796509488907No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC292870.20083732163232665No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG286170.19624275730366006No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG283370.19432264086779938No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA276910.18989265794792082No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA265620.1821504741761826No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA240350.16482142334254005No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG235250.16132406840579386No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT222190.1523680967442437No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC219820.15074285533246165No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC216910.14874730575090644No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA214790.14729350330661195No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA213060.14610714565159802No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC207990.1426303633909503No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT206970.14193089240360107No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA205920.14121084874015333No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT201960.13849525549515038No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG199890.13707574084435337No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA194090.133098356798642No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT191290.1311782403627813No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA187240.12840092908948283No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC184380.12643966730142517No Hit
CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTC183780.12602821377945503No Hit
CCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCCCCAAGACCTC182070.12485557124184012No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC181860.12471156250915055No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG178650.12251028616661029No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT177830.12194796635325109No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT173610.11905407658206109No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT172150.11805287301193373No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA170040.11660592812633873No Hit
GCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAG166740.11434293375550293No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG164910.11308800051349399No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG164850.11304685516129699No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC164050.1124982504653368No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC162940.11173706144969203No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA162720.11158619515830297No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA160870.11031754679889502No Hit
CTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCA158790.10889117458939854No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC155920.10692305524264134No Hit
CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC154030.1056269766484354No Hit
CACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGG153320.10514008998077072No Hit
CTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTG153280.10511265974597273No Hit
CTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAG151560.1039331596496583No Hit
GTGATGTTATAAGCCTAAGGCAGCTTGACTTGCAGCAACAAGTTTTTTAC146090.1001820750410305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)