FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004829179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004829179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14863852
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA322560.21700969573701354No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT274710.18481750221947849No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT241780.16266308356676318No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG190630.12825073877215679No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC185810.12500797236140404No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA162890.10958801258247189No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG160590.10804063441966456No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG157310.10583393860487847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT61500.041.4340194
ACCCGGC72400.038.4020165
CGACCGT12500.038.3228761
CACCCGG75150.036.6841554
TCACCCG77250.035.8691833
CGACCTA17500.033.5460474
TAGGTCG16050.029.2612533
CTCGATT56550.028.84402348-49
ACCGTTG17400.028.0706213
ATTCCGT61300.026.72578652-53
CACGCCG40650.026.457234
CGGACAC65500.025.45582412-13
CGGCCCG110200.025.442818
TCGTAAG13800.025.1838476
GACCGTT19300.025.0638562
TTCTCGA66850.024.68098346-47
TGACGAC25350.024.454791
CGCGGTC14450.024.375941
CGTTCTT65400.024.30121876-77
CTTAGCG15950.024.1449058
Produced by FastQC (version 0.11.5)